The mechanism of apoptosis induced by homoharringtonine in HL-60 cells

AIM: To study the signal transduction pathway of apoptosis initiation induced by homoharringtonine in HL-60 cells. METHODS: After establishing the model of apoptosis initiation induced by homoharringtonine in HL-60 cells, at the point of apoptosis initiation, molecular caspase-3, Bcl-2, Bax and Fas/FasL were measured with flow cytometry and transmission electron microscope. ERK2 and P38 expression in HL-60 cells were detected by using immunohistochemistry. RESULTS: The model of apoptosis initiation induced by homoharringtonine was established in HL-60 cells. At the point of apoptosis initiation, upregulation of caspase-3 and decrease in Bcl-2/Bax were observed. However, the expression of Fas/FasL did not significantly change. ERK2 expression decreased and P38 expression increased. CONCLUSIONS: Caspase-3, Bcl-2, Bax and mitogen activated protein kinase pathways were involved in signal transduction of apoptosis initiation induced by homoharringtonine in HL-60 cells.     

Effect of atrial natriuretic peptide on acute lung injury induced by lipopolysaccharide

AIM: To investigate effect of atrial natriuretic peptide (ANP) on acute lung injury (ALI) induced by lipopolysaccharide (LPS) in rat. METHODS: Mean arterial blood pressure (MAP) was recorded with model 6280 physiology intelligentialize grapher, nitric oxide (NO) and endothelin (ET) concentrations in plasma were measured after lipopolysaccharide (LPS) or following LPS ,ANP was injected into vein in rats. After experiment,lung water as well as pulmonary histopathological changes was measured and observed, respectively. RESULTS: Administration of LPS elicited a persistence decrease in MAP (8.1 kPa±2.6 kPa,at 4 h,P<0.01 vs control); NO and ET concentration in plasma was evident higher than that in control group, respectively (P<0.01); Wet-dry ratio of lung was higher than that in control group (5.15±0.43,at 4 h) (P<0.05); Alveolus detelectasis was observed and pulmonary mesenchyme was thicker than that in control group. No erythrocyte and leukocytes in alveolus,which show an interstitial pulmonary edema, was observed in LPS+ANP group, ANP maintained MAP at higher levels (13.35 kPa±2.93 kPa, at 4 h, P<0.05 vs LPS) after an transient decline when LPS was injected; NO and ET concentration of plasma had all significantly decrease, respectively (P<0.05 vs LPS, at 4 h); Wet-dry ratio of lung was lower than LPS group (4.57±0.35, P<0.05). Compared with control group the ratio was not evident difference (P>0.05); The histopathological of lung displayed markedly improved. CONCLUSION: ANP attenuates ALI induced by LPS in the rat. The effect of ANP may be via decreasing secretion of ET,NO and regulation arterial blood pressure.     

体外试验中不同粗精比对山羊瘤胃细菌氨态氮利用效率的影响

利用体外发酵法研究了4种粗精比(10∶0、8∶2、6∶4和4∶6)底物对山羊瘤胃细菌产量、15NH3-N利用效率的影响。结果表明:混合培养液的pH值与氨态氮浓度在8h内急剧下降,而后至24h呈缓慢下降;经24h培养的细菌产量在8∶2、6∶4和4∶6组中差异不显著(P>0.05),但都极显著地高于10∶0组(P<0.01);细菌体15N丰度以8∶2及4∶6组较高,显著高于10∶0和6∶4组(P<0.05);细菌体15N富集量以8∶2组最高,底物547.43μg15N中有120.44μg被细菌体富集,显著高于10∶0组(P<0.05),但6∶4、4∶6组与10∶0组间差异均不显著(P>0.05)。细菌赖氨酸的相对百分组成随精饲料比例的增加而显著增加,胱氨酸在粗饲料组(10∶0)显著高于其它组,但各精饲料添加组间差异不显著,组氨酸以8∶2组为最高,显著高于10∶0组,但与其它精饲料添加组间差异不显著(P>0.05)。     

ob基因及其表达产物的生物学效应

ob基因自从被克隆后,围绕其表达产物的作用机理和生物学效应进行了大量的研究,最近研究证明Leptin对小鼠大脑发育有重要功能,标志着Leptin的研究进入了一个新的阶段,今后将从单一的营养调控研究转向兼顾神经生物学的研究,更深入研究Leptin作用机理。同时国内外学者从分子领域探究ob基因在畜禽育种上的作用,做了很多的工作,也取得了重要的成果。本文总结了ob基因的重要生物学效应,为理解ob基因的作用机制和进一步研究人类肥胖治疗和提高动物生长繁殖性状水平提供必要的支持。     

鸡传染性喉气管炎油乳剂灭活疫苗的研究

分别将3株鸡传染性喉气管炎病毒(标准强毒株J株、山东分离株N株和L株)经甲醛灭活后制成油乳剂灭活疫苗。免疫效力试验结果表明，以N株鸡传染性喉气管炎病毒制备的油乳剂灭活疫苗免疫效果较其它几种疫苗好，免疫鸡AGP抗体阳性率高达83％，免疫保护率高达100％。     

猪附红细胞体感染对仔猪猪瘟免疫效果的影响

为探讨猪附红细胞体对仔猪猪瘟免疫效果的影响,本试验利用猪瘟抗体检测ELISA试剂盒,对已注射猪瘟疫苗的69头感染猪附红细胞体的仔猪和31头无猪附红细胞体感染的健康仔猪进行了猪瘟抗体检测。结果表明,感染猪附红细胞体仔猪的猪瘟抗体水平低下,其猪瘟疫苗整体免疫合格率(49·2%)明显低于健康仔猪(93·5%),且显性感染仔猪的免疫合格率(41·6%)明显低于隐性感染仔猪(53·5%)。说明猪附红细胞体严重干扰了猪瘟疫苗的免疫效果,且干扰程度与猪附红细胞体的感染程度呈正相关。     

新疆干旱地区紫花苜蓿种植技术要点

近年来新疆干旱区紫花苜蓿种植面积基本呈逐年上升趋势,苜蓿在各地区的种植业结构调整中已占据重要位置,成为农牧结合的纽带.作者在新疆喀什地区对紫花苜蓿新品种进行多年的种植试验示范,积累了干旱区苜蓿种植的实践经验.本文力图从重新认识和重视苜蓿生产的作用出发,强化农艺实用技术的示范推广和对其生产者的指导与服务,切实提高苜蓿生产的经济效益.     

川西北高寒沙地不同年限高山柳林下优势植物碳、氮、磷生态化学计量特征

通过采集川西北高寒沙地不同年限(6、18、24年)高山柳林下3种优势植被藏沙蒿、裂叶独活和镰荚棘豆,分别测定分析3种植被叶片、根部C、N、P化学计量特征变化特征。结果表明:不同年限高山柳林下植被C、N、P含量及其比值间存在显著差异且呈现出不同变化趋势。林下植被C含量整体下降;叶N含量呈上升趋势,根N含量随年限增长而下降;除藏沙蒿外,林下植被P含量变化不显著;C∶N变化范围为1.92~12.86;C∶P为29.18~196.88;不同年限高山柳林下植被N∶P间虽存在差异,但均表现出主要受到P限制,表明该区域植被生长主要受P限制,应注意P养分的适当补充。     

Investigation of Clinical Medicine for Treating Cows Endometritis in Baotou Area

In 4 dairy farms located in Baotou area, clinical medicine investigation and drug resistance of pathogenic bacteria were conducted, and then curative effects of different treatment methods were analyzed.Epidemic materials were collected from diary cows infected by endometritis, from which pathogenic bacteria were isolated using conventional microbiology technologies.Broth microdilution method was used to detect the sensitivity and resistance of pathogenic bacteria to antibiotics commonly used in clinic.On this basis, the sensitive drugs were used to treat mastitis or endometritis, the clinical curative effect of traditional treatment group was compared with that of therapeutic test group.The result of traditional treatment group showed 13 antibiotics were used to treat 160 cases of endometritis, florfenicol was most effective, penicillin was most ineffective.The results of therapeutic test group showed that Staphylococcus aureus, Escherichia coli and Streptococcus were the main pathogenic bacteria of endometritis, which were sensitive to gentamicin and cefalotin, etc.The results of statistical analysis showed that the curative effect of therapeutic test group was extremely significantly different from that of traditional treatment group(P<0.01).Sensitive drugs screened by drug sensitive test could be used to target therapy, and obtain satisfied curative effect.Moreover, this approach could avoid antibiotic abuse.     

靖边县2010年—2012年羊布鲁菌病血清学调查

为了解陕西省靖边县羊布鲁菌的感染及分布状况,自2010年1月—2012年12月,采集陕西省靖边县羔羊、种羊等55 059只的羊血清样品,通过试管凝集反应对布鲁菌病抗体进行检测,分析羊布鲁菌的分布情况。结果显示,55 059份羊血清样品共检出阳性样品78份,其中羔羊样品阳性71份。种羊养殖场和养殖户因引进布鲁菌隐性种公羊造成2个养殖场出现77只病羊,4个养殖户出现117只病羊。羔羊布鲁菌抗体阳性率呈增高趋势,由2010年的0.04%上升到2012年的0.29%;由于引种造成的布鲁菌感染率较高。